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Cholesterol Kit is a safe, convenient and cost-effective way of testing the cholesterol level. This is developed for monitoring the cholesterol levels with high accuracy in not-time. This is a simple method to quantify the esters, total and free cholesterol in samples. It is also provided with easy separation methods for LDL or VLDL and HDL cholesterol. Cholesterol oxidase is used to act on free cholesterol for developing a chemical so that it can reach with a probe and develop color (570 nm) and fluorescence. Cholesterol esterase is utilized for hydrolyzing cholesteryl ester into free cholesterol.