The Leishmania ELISA kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect specific antibodies against Leishmania parasites in a sample. The principle involves coating microtiter plates with Leishmania antigens, adding the sample, and then adding an enzyme-labeled antibody specific for Leishmania antibodies. After washing away unbound reagents, a substrate is added that reacts with the enzyme, producing a color change proportional to the amount of Leishmania antibodies present in the sample.