About Bilirubin Direct (Liquid)
Sulfanilic acid reacts with sodium nitrite to form diazotized Sulfanilic acid. In the presence of accelerator (cetrimide), conjugated and unconjugated bilirubin reacts with diazotized Sulfanilic acid to form azobilirubin (Bilirubin Total). In the absence of accelerator only conjugated bilirubin reacts (Bilirubin Direct). The increase of absorbance at 546 nm is proportional to bilirubin concentration.
Ready-to-Use ConvenienceBilirubin Direct (Liquid) streamlines laboratory operations as a ready-to-use reagent. With no mixing or preparation required, laboratory professionals can directly integrate it into serum or plasma bilirubin testing, ensuring efficiency and reliability in routine diagnostics.
Optimized Stability & StorageThe reagent remains effective up to its stated expiry date when stored at recommended temperatures (2C8C). The robust plastic bottle packaging preserves its high sensitivity and prevents contamination, so users can maintain consistent quality throughout its shelf life.
High Sensitivity With Minimal InterferenceSpecially formulated to minimize sample interference, Bilirubin Direct (Liquid) ensures accurate colorimetric detection of bilirubin. Its optimized pH range and stability are ideal for clinical laboratories seeking dependable and reproducible results during bilirubin analysis.
FAQs of Bilirubin Direct (Liquid):
Q: How should Bilirubin Direct (Liquid) be stored to maintain its effectiveness?
A: Always store Bilirubin Direct (Liquid) between 2C and 8C in its original plastic bottle. Proper refrigeration ensures the reagent remains stable and effective up to its expiry date.
Q: What is the main application of Bilirubin Direct (Liquid) in clinical laboratories?
A: This reagent is used primarily for the quantitative determination of bilirubin levels in serum or plasma samples, providing essential data for liver function assessments and diagnostic procedures.
Q: When can Bilirubin Direct (Liquid) be used after opening the bottle?
A: Once opened and handled under proper laboratory conditions, the reagent remains stable and usable until the stated expiry, as long as recommended storage temperatures are maintained.
Q: Where should the reagent be kept within the laboratory for optimal stability?
A: For best preservation, store the Bilirubin Direct (Liquid) reagent in a dedicated laboratory refrigerator between 2C and 8C, away from direct sunlight and heat sources.
Q: What is the process for using Bilirubin Direct (Liquid) in bilirubin determination procedures?
A: Simply follow the laboratorys established colorimetric assay protocol, using Bilirubin Direct (Liquid) directly from the bottle. No additional mixing is necessary, as it is supplied ready-to-use.
Q: How does the formulation of Bilirubin Direct (Liquid) benefit laboratory testing?
A: Its high sensitivity and minimized interference enhance result accuracy, while its ready-to-use format speeds up workflow and reduces the risk of error during preparation.