About CK-MB (Liquid)
Human CK-MB is composed of two subunits, CK-M and CK-B which both have
an active site. With the aid of specific antibodies to CK-M, the catalytic activity
of CK-M subunits in the sample is inhibited to 99.6 % without affecting the
CK-B subunits. The remaining CK-B activity, corresponding to half the CK-MB
activity, is determined by the total CK method. As the CK-BB isoenzyme
only rarely appears in serum and the catalytic activity of the CK-M and CK-B
subunits hardly differ, the catalytic activity of the CK-MB isoenzyme can be
calculated from the measured CK-B activity by multiplying the result by 2.
Ready-to-Use, Hassle-Free DetectionCK-MB (Liquid) eliminates preparation complexity by providing a single liquid reagent, ready for direct application. Theres no need for time-consuming calibrations, allowing for straightforward workflow integration in both high-throughput and low-volume laboratories. This simplicity ensures consistency and saves valuable technician time.
Reliable and Versatile PerformanceThe assays compatibility with all semi-automatic and fully automatic analyzers makes it suitable for a broad range of laboratory environments. Utilizing the IFCC-approved enzymatic UV kinetic method, it delivers results with high specificity and accuracy across diverse clinical settings.
Extended Shelf Life and Stable StorageWith an 18-month shelf life from the manufacturing date and stability assured at 2C 8C, CK-MB (Liquid) minimizes reagent wastage. This makes it an economical choice for laboratories needing reliable, long-lasting reagents for cardiac marker testing.
FAQs of CK-MB (Liquid):
Q: How is the CK-MB (Liquid) reagent used for CK-MB determination?
A: Simply add 20 L of serum or plasma sample to the reaction mixture as instructed. Incubate at 37C for 5 minutes, and the reagent will measure CK-MB activity using the UV kinetic method as per IFCC guidelines.
Q: What advantages does the single liquid, ready-to-use format offer?
A: This format removes the need for reagent preparation or calibration, saving time and reducing the possibility of errors, while streamlining the testing process for laboratory staff.
Q: When should the CK-MB (Liquid) reagent be used in the diagnostic process?
A: CK-MB (Liquid) should be used when a quantitative determination of CK-MB enzyme activity is needed, typically in the evaluation of suspected myocardial injury in clinical diagnostics.
Q: Where should this reagent be stored, and how long is it stable?
A: Store CK-MB (Liquid) at a temperature between 2C and 8C. The reagent remains stable and effective for up to 18 months from the date of manufacturing, when stored as directed.
Q: What sample and reaction volumes are required for analysis?
A: The method requires a sample volume of just 20 L and a total reaction volume of 1000 L for each determination.
Q: Is this reagent compatible with all clinical laboratory analyzers?
A: Yes, CK-MB (Liquid) is designed for seamless use with both semi-automatic and fully automatic analyzers, offering flexible application across various laboratory platforms.
Q: What benefits does this assay method provide to laboratories?
A: It offers fast turnaround times (results in 5 minutes), no requirement for calibration with ready-to-use packs, and reliable quantitative analysis, improving efficiency and ensuring accuracy in cardiac diagnostics.