Follicle Stimulating Hormone (FSH) Kit
Product Code : BGEFSH
Brand Name : Biogenix
The BI-EIA-GONADOTROPIN-FSH is a one-step immunoassay, based on principle of sandwich method. The assay system utilizes a high affinity andspecificity monoclonal antibody (enzyme conjugated and immobilized) directed againsta distinct antigenic determinant on the intact FSH molecule. The test sample isallowed to react simultaneously with the two antibodies, resulting in the FSHmolecules being sandwiched between the solid phase and enzyme-linkedantibodies. After incubation, the wells are washed with washing solution toremove unbound labeled antibodies. A solution of TMB-Substrate is added andincubated, resulting in the development of a blue color. The color developmentis stopped with the addition of Stopping Reagent, changing the color to yellow.The concentration of FSH is directly proportional to the color intensity of thetest sample. Absorbance is measured spectrophotometrically at 450 nm.