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Thyroid Hormones - T3 Total Kit
The âBI-EIA-Thyroid-T3 totalâ is a one-step immunoasay to determine the presence of totaltriiodothyronine (total T3) in human serum using competitive microplate enzymeimmunoassay. Platesare coated with anti-T3 antibodies.Serum reference, patient specimens or control serum is first added tomicroplate well. Enzyme-T3 conjugate is added. A competition reaction resultsbetween the enzyme-triiodothyronine conjugate and a serum containing the nativetotal triiodothyronine for a limited number of antibody combining sitesimmobilized on the well. Unboundconjugate is removed by washing. The enzyme activity in the antibody-bound fraction isinversely proportional to the native total triiodothyronine concentration. Theenzyme activity is revealed by a color change in TMB-substrate solution.