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Thyroid Hormones- TSH Kit
Product Code : BGETSH
Brand Name : Biogenix
The BI-EIA-Thyroid-TSH is a one-step immuno assay, based on principle of sandwich method. The assay system utilizes a high affinity and specificity monoclonal antibodies (enzymeconjugated and immobilized) directed against a distinct antigenic determinant on the intact TSH molecule. The test sample is allowed to react simultaneously with the two antibodies, resulting in the TSH molecules being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed with washing buffer to remove unbound labeled antibodies. Asolution of TMB - Substrate is added and incubated, resulting in the developmentof a blue color. The color development is stopped with the addition of StoppingReagent, changing the color to yellow. The concentration of TSH is directly proportional to the color intensity of the test sample. Absorbance is measured spectro photo metrically at 450 nm.