TOTAL PROTEIN (Liquid)

TOTAL PROTEIN (Liquid) Specification

Intended Use
Quantitative Determination of Total Protein in Serum and Plasma

Reference Range
6.0 8.0 g/dL (adults)

Sample Type
Serum, Plasma

Product Name
TOTAL PROTEIN (Liquid)

Sample Volume
Typically 10 L per test

Measuring Wavelength
546 nm

Pack Size
5 x 100 ml

Reading Stability
Stable for 30 minutes at 20-25C after mixing

Linearity
Up to 15 g/dL

Storage Temperature
2-8C

Calibration
Needs recalibration after reagent lot change

Shelf Life
Up to expiry date mentioned on the label when stored at recommended condition

Reagent Volume
1.0 mL per test

Sensitivity
Can detect as low as 0.10 g/dL protein level

Controls
Use appropriate control sera

Method Compatibility
Automated and manual applications

Reagent Type
Liquid, ready-to-use

Interference
Hemolysis, lipemia, and icterus may interfere

Principle
Biuret method

TOTAL PROTEIN (Liquid) Trade Information

Minimum Order Quantity
1000 Milliliters

Sample Policy
If order is confirmed we will reimburse the sample cost

Main Export Market(s)
Asia, Central America, South America, Eastern Europe, Western Europe, Africa

Main Domestic Market
All India

About TOTAL PROTEIN (Liquid)

Protein in an alkaline medium, bind with the cupric ions presents in biuret reagent to form a blue violet coloured complex. The intensity of the colour formed is directly proportional to the amount of protein present in the sample.

Accurate and Reliable Protein Quantitation

TOTAL PROTEIN (Liquid) employs the trusted biuret method for precise measurement of total protein in serum and plasma samples. The reagent is optimized to deliver sensitive and linear results, making it suitable for use in both routine and specialized laboratory workflows. Laboratories benefit from its ease of use and robust performance.

User-Friendly, Ready-to-Use Reagent

This liquid reagent is ready-to-use, minimizing preparation time and potential errors associated with mixing. Delivered in convenient 5 x 100 ml packs, it allows quick and efficient processing without sacrificing accuracy. Automation and manual compatibility further ensure versatile integration into laboratory systems.

Designed for Stability and Consistency

The stable formulation allows for accurate readings within 30 minutes of mixing at room temperature (20-25C). Careful design ensures consistent results across batches when recalibrated with lot changes. Store TOTAL PROTEIN (Liquid) at 2-8C to preserve its integrity until the expiration date.

FAQs of TOTAL PROTEIN (Liquid):

Q: How is the TOTAL PROTEIN (Liquid) test performed?

A: The test uses the biuret method and requires 10 L of serum or plasma sample and 1.0 mL of reagent per test. After mixing, the protein content is measured at a wavelength of 546 nm, either using automated analyzers or manual equipment.

Q: What is the sensitivity and linearity range of the reagent?

A: TOTAL PROTEIN (Liquid) is sensitive enough to detect protein levels as low as 0.10 g/dL. It maintains linearity for measurements up to 15 g/dL, ensuring accurate results across the clinically relevant range.

Q: When should recalibration be performed?

A: Recalibration must be done whenever there is a reagent lot change to ensure continued accuracy. Use the recommended calibration procedures with each new batch of reagent.

Q: What storage conditions are required for this product?

A: The reagent should be stored at temperatures between 2-8C. Under these conditions, it remains stable and usable up to the expiry date shown on the product label.

Q: Does the procedure require any controls during testing?

A: Yes, it is important to use appropriate control sera for each run to validate the accuracy and reliability of results obtained with the reagent.

Q: What interferences can affect the assay results?

A: Hemolysis, lipemia, and icterus in the sample may interfere with protein measurements. It is recommended to check samples for these conditions before testing to avoid inaccuracies.