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H. Pylori Ab
The H. pylori Ab Combo Rapid Test is a lateralflow chromatographic immunoassay based on the principle of the double-antigensandwich technique. The test cassette consists of: 1) a burgundy coloredconjugate pad containing H. pylori antigens including Cag-A conjugated withcolloidal gold (H. pylori conjugates) and a control antibody conjugated withcolloidal gold, and 2) a nitrocellulose membrane strip containing a test line(T line) and a control line (C line). The T line is pre-coated withnon-conjugated H. pylori antigens, and the C line is pre-coated with a controlline antibody.
When anadequate volume of test specimen is dispensed into the sample well of thecassette, the specimen migrates by capillary action across the cassette.Antibodies (IgG, IgM or IgA) to H. pylori, if present in the specimen, willbind to the H. pylori conjugates. The immunocomplex is then captured on themembrane by the pre-coated H. pylori antigens forming a burgundy colored Tline, indicating a H. pylori Ab positive test result. Absence of the T linesuggests a negative result.Thetest contains an internal control (C line) which should exhibit a burgundycolored line of the immunocomplex of the control antibodies regardless of colordevelopment on the T line. If the C line does not develop, the test result isinvalid, and the specimen must be retested with another device.