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Hepatitis A Virus IgM (HAV IgM) ELISA Kit

Hepatitis A Virus IgM (HAV IgM) ELISA Kit Specification

Accuracy
High Sensitivity and Specificity

Control Type
Manual (with positive and negative controls provided)

Features
Ready-to-use reagents, colorimetric detection, high accuracy, includes controls, microplate format

Temperature Resistance
Store at 2-8C

Shape
Rectangular (microplate format)

Type
ELISA Kit

Equipment Type
ELISA Diagnostic Kit

Equipment Materials
Plastic, polystyrene microplate, reagent bottles

Material
High-grade laboratory plastics and reagents

Application
Qualitative detection of IgM antibodies to Hepatitis A Virus in human serum or plasma

Intended User
Professional users/laboratory use only

Format
96 wells/plate

Detection Method
Enzyme-Linked Immunosorbent Assay (ELISA), Sandwich Principle

Assay Type
Qualitative

Incubation Time
Approximately 60-75 minutes

Specificity
>99%

Control Included
Positive and Negative Controls included

Kit Components
Pre-coated microplate, conjugate, substrate, stop solution, wash buffer, sample diluent, controls

Sensitivity
>99%

Regulatory Status
For in vitro diagnostic use only (IVD)

Sample Volume Required
100 L per well

Reading Wavelength
450 nm (reference 620 nm)

Shelf Life
12-18 months when stored at 2-8C

Hepatitis A Virus IgM (HAV IgM) ELISA Kit Trade Information

Minimum Order Quantity
1 , , Kit

Payment Terms
Cash in Advance (CID), Cash Advance (CA)

Supply Ability
50000 Kits Per Week

Delivery Time
7 Days

Main Domestic Market
All India

About Hepatitis A Virus IgM (HAV IgM) ELISA Kit

This assay is based upon the two-step capture method. In the first step, the sample and mouse monoclonal Anti-human IgM coated micro plate are combined. During the incubation, IgM antibodies present in the sample bind to the Anti-human IgM coated on the wells. After the washing, in the second step, HAV antigens and Enzyme Conjugate are added to the wells. During the incubation, HAV antigens are allowed to react with the Anti-HAV IgM bound to the micro plate and enzyme labeled antibodies, then a complex is generated among the solid phase, HAV antigens and enzyme-linked antibodies by immunological reactions. After a second washing, Substrate A and Substrate B are then added and catalyzed by this complex, resulting in a chromogenic reaction. The resulting chromogenic reaction is measured as absorbance. The color intensity is proportional to the amount of Anti-HAV in the sample.

Superior Diagnostic Accuracy

The HAV IgM ELISA Kit boasts high sensitivity and specificity, making it a dependable choice for detecting recent Hepatitis A infections. With an accuracy rate exceeding 99%, this kit ensures reliable results that enhance laboratory diagnosis and patient management.

Simplified Workflow and Rapid Results

With ready-to-use reagents and a streamlined 6075 minute protocol, this kit makes diagnosis efficient for laboratory professionals. The microplate format allows multiple samples to be processed simultaneously, enhancing throughput in busy diagnostic settings.

Clearly Defined Controls and User Assurance

Both positive and negative controls are included in each kit, supporting manual quality monitoring and result validation. This inclusion strengthens the accuracy and reliability of each assay, making it ideal for professional laboratory use.

FAQs of Hepatitis A Virus IgM (HAV IgM) ELISA Kit:

Q: How does the HAV IgM ELISA Kit detect Hepatitis A infections?

A: The kit utilizes the sandwich ELISA principle to capture and identify IgM antibodies specific to Hepatitis A Virus in human serum or plasma, providing qualitative results via colorimetric detection at 450 nm.

Q: What sample volume is needed for each test well?

A: Each test requires 100 L of serum or plasma per well. This volume ensures precise and reproducible qualitative results.

Q: When should I use this kit for testing?

A: Use this kit when there is a clinical suspicion of recent or acute Hepatitis A infection. It is particularly effective during the early symptomatic phase when IgM antibodies typically appear.

Q: Where should the HAV IgM ELISA Kit be stored for optimal stability?

A: To maintain reagent integrity and maximize shelf life (1218 months), store the kit at a temperature between 28C in a laboratory refrigerator.

Q: What is the typical process for running the ELISA assay?

A: The process involves adding pre-diluted samples and controls to the pre-coated microplate, followed by incubations, washes, addition of the conjugate and substrate, and a final stop solution addition before measuring absorbance.

Q: Who should use this ELISA Kit?

A: This kit is intended for professional use by trained laboratory personnel only, as it is classified for in vitro diagnostic applications and requires laboratory-specific equipment and handling.

Q: What are the key benefits of this ELISA kit for laboratories?

A: Laboratories benefit from high accuracy, ease of use with ready-to-use reagents, included controls for result validation, and efficient assay completion within approximately 6075 minutes.

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