About TOTAL PROTEIN (Liquid)
Protein in an alkaline medium, bind with the cupric ions presents in biuret reagent to form a blue violet coloured complex. The intensity of the colour formed is directly proportional to the amount of protein present in the sample.
Accurate and Reliable Protein QuantitationTOTAL PROTEIN (Liquid) employs the trusted biuret method for precise measurement of total protein in serum and plasma samples. The reagent is optimized to deliver sensitive and linear results, making it suitable for use in both routine and specialized laboratory workflows. Laboratories benefit from its ease of use and robust performance.
User-Friendly, Ready-to-Use ReagentThis liquid reagent is ready-to-use, minimizing preparation time and potential errors associated with mixing. Delivered in convenient 5 x 100 ml packs, it allows quick and efficient processing without sacrificing accuracy. Automation and manual compatibility further ensure versatile integration into laboratory systems.
Designed for Stability and ConsistencyThe stable formulation allows for accurate readings within 30 minutes of mixing at room temperature (20-25C). Careful design ensures consistent results across batches when recalibrated with lot changes. Store TOTAL PROTEIN (Liquid) at 2-8C to preserve its integrity until the expiration date.
FAQs of TOTAL PROTEIN (Liquid):
Q: How is the TOTAL PROTEIN (Liquid) test performed?
A: The test uses the biuret method and requires 10 L of serum or plasma sample and 1.0 mL of reagent per test. After mixing, the protein content is measured at a wavelength of 546 nm, either using automated analyzers or manual equipment.
Q: What is the sensitivity and linearity range of the reagent?
A: TOTAL PROTEIN (Liquid) is sensitive enough to detect protein levels as low as 0.10 g/dL. It maintains linearity for measurements up to 15 g/dL, ensuring accurate results across the clinically relevant range.
Q: When should recalibration be performed?
A: Recalibration must be done whenever there is a reagent lot change to ensure continued accuracy. Use the recommended calibration procedures with each new batch of reagent.
Q: What storage conditions are required for this product?
A: The reagent should be stored at temperatures between 2-8C. Under these conditions, it remains stable and usable up to the expiry date shown on the product label.
Q: Does the procedure require any controls during testing?
A: Yes, it is important to use appropriate control sera for each run to validate the accuracy and reliability of results obtained with the reagent.
Q: What interferences can affect the assay results?
A: Hemolysis, lipemia, and icterus in the sample may interfere with protein measurements. It is recommended to check samples for these conditions before testing to avoid inaccuracies.
Q: What is the reference range for total protein in adults using this reagent?
A: The reference range for total protein in adult serum or plasma samples is 6.0 8.0 g/dL, as measured with the TOTAL PROTEIN (Liquid) reagent.