Anti CCP ELISA Kit

Anti CCP ELISA Kit Specification

Shape
Rectangular microplate

Control Type
Manual, as per ELISA protocol

Features
Ready-to-use reagents, high precision, reliable results, easy-to-follow instructions

Accuracy
High sensitivity and specificity (>95%)

Temperature Resistance
Store at 2-8C

Type
Anti CCP ELISA Kit

Equipment Type
Enzyme Immunoassay (ELISA) Kit

Equipment Materials
High-quality polystyrene microplate, reagents in plastic vials

Material
Reagents, Enzyme conjugate, Standard controls, Polystyrene plate

Application
Detection of Anti-Cyclic Citrullinated Peptide (CCP) antibodies in human serum or plasma

Anti CCP ELISA Kit Trade Information

Minimum Order Quantity
1 Kit

Payment Terms
Cash Advance (CA)

Sample Policy
If order is confirmed we will reimburse the sample cost

Main Domestic Market
All India

About Anti CCP ELISA Kit

The Anti-CCP assay is a two-step immunoassay with an automated sample pretreatment for the semi-quantitative determination of the IgG class of auto antibodies specific to cyclic citrullinated peptide(CCP) in human serum or plasma using CMIA technology with flexible assay protocols, referred to as Chemiflex.In the first step, sample is prediluted with wash buffer. The prediluted sample, CCP coated paramagnetic microparticles, and sample diluentare combined. Anti-CCP antibodies present in the sample bind to the CCPcoated micro particles. After washing, anti-human IgG acridinium-labeled conjugate is added in the second step. Following another wash cycle,pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units(RLUs). A direct relationship exists between the amount of anti-CC Pantibody in the sample

Reliable Detection Method

Utilizing a colorimetric detection measured at 450 nm, the Anti CCP ELISA Kit ensures precise quantification of anti-CCP antibodies. The kits high-quality reagents and polystyrene microplate contribute to consistent and reproducible outcomes, making it suitable for clinical and research laboratories.

Simple and Efficient Workflow

Each assay requires only 10 L of serum or plasma, optimizing sample conservation. The kit arrives with all required reagents premixed and easy-to-follow instructions, streamlining your laboratory workflow and minimizing pipetting steps to reduce errors.

Long Shelf Life & Convenient Storage

The kit remains stable for 12 to 18 months when stored at 2-8C and protected from light. This extended shelf life allows for flexible inventory management and ensures kits remain usable over multiple testing cycles without degradation in performance.

FAQs of Anti CCP ELISA Kit:

Q: How should the Anti CCP ELISA Kit be stored to maintain its stability?

A: Store the kit at 2-8C, protected from light, to preserve reagent integrity and ensure reliable results throughout the 12-18 month shelf life.

Q: What process is followed when using the Anti CCP ELISA Kit?

A: Begin by adding 10 L of patient serum or plasma to the microplate wells, then proceed according to the supplied instruction manual. The protocol includes enzyme conjugation, substrate development, and colorimetric measurement at 450 nm, with total assay time around 2 hours.

Q: What samples can be used and how much is required per test?

A: This kit is compatible with human serum or plasma samples, requiring only 10 L per well for each individual test.

Q: What are the major benefits of using this kit in a diagnostic setting?

A: The kit offers high sensitivity and specificity, ready-to-use reagents, a short assay time, and easy-to-follow instructions, all contributing to trustworthy and consistent diagnostic results for anti-CCP antibodies.

Q: Where is this ELISA kit manufactured and supplied?

A: The Anti CCP ELISA Kit is manufactured, exported, and supplied from India, with compliance to CE marking for international standards.

Q: When can you expect to receive results after starting the assay?

A: Results are typically available in about 2 hours, making it suitable for timely diagnostic decision-making in clinical laboratories.

Q: What calibration method is used in the kit for accurate antibody quantification?

A: Standard calibration curves are provided with the kit, allowing for precise quantification of anti-CCP antibody levels in patient samples according to established ELISA techniques.

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