Anti CCP ELISA Kit

The Anti-CCP assay is a two-step immunoassay with an automated sample pretreatment for the semi-quantitative determination of the IgG class of auto antibodies specific to cyclic citrullinated peptide(CCP) in human serum or plasma using CMIA technology with flexible assay protocols, referred to as Chemiflex.In the first step, sample is prediluted with wash buffer. The prediluted sample, CCP coated paramagnetic microparticles, and sample diluentare combined. Anti-CCP antibodies present in the sample bind to the CCPcoated micro particles. After washing, anti-human IgG acridinium-labeled conjugate is added in the second step. Following another wash cycle,pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units(RLUs). A direct relationship exists between the amount of anti-CC Pantibody in the sample