SYPHILIS Ab RAPID TEST (W/B)

After addition of the serum or plasma sample to the sample well of the device containing a test strip, the sample moves on to the conjugate pad containing colloidal gold particles conjugated with recombinant Treponema pallidum specific antigens (15,17,47 kDA) and Mouse IgG. If the sample contains detectable levels of the Treponema pallidum antibodies, it reacts with the gold conjugated recombinant Treponema pallidum specific antigens to form a complex. This complex moves further and Treponema pallidum specific antibodies conjugate complex reacts with recombinant Treponema pallidum specific antigens (15,17,47 kDA) test line on the nitrocellulose membrane area to form colored band. The unbound complex and the mouse IgG conjugated colloidal gold particles move further to the goat anti-mouse IgG coated control area to form a colored band (Control line). The appearance of test line and control line in respective area indicates the positive result. Appearance of only control line indicates a negative result. The control line acts as a procedural control. Control line should always appear if the test is performed as per the procedure and reagents are working properly.