Progesterone ELISA Kit

Progesterone ELISA Kit Specification

Temperature Resistance
Store at 2-8C

Control Type
Manual (microplate reader required)

Accuracy
Typically <10% intra- and inter-assay CV

Features
Ready-to-use reagents; High sensitivity and specificity; User-friendly protocol

Type
ELISA Kit

Dimension (L*W*H)
Standard Kit Packaging

Equipment Type
Hormone Assay ELISA Kit

Equipment Materials
Kit comprises pre-coated 96-well plate, buffers, reagents, substrate solution, conjugate, standard solution

Material
High-grade plastic, reagents, buffers

Application
Quantitative determination of Progesterone in serum/plasma

Progesterone ELISA Kit Trade Information

Minimum Order Quantity
1 , , Kit

Supply Ability
10000 Kits Per Week

Delivery Time
7 Days

Sample Policy
If order is confirmed we will reimburse the sample cost

Main Export Market(s)
Middle East, North America, Central America, South America, Australia, Western Europe, Eastern Europe, Africa, Asia

Main Domestic Market
All India

About Progesterone ELISA Kit

Progesterone is a one-step immunoassay, based on the principle of the competitive method. Progesterone present in the sample and the labeled enzyme-progesterone in the conjugate compete for binding to the capture antibody on the anti-progesterone coated micro plate. The en- zyme activity in the antibody-bound fraction is inversely proportional to the native progesterone concentration.The unbound components are removed by washing. After addition of the solution containing TMB and hydrogen peroxide, the wells with bound conjugate develop a blue color which is converted to yellow after the reaction has been stopped with sulphuric acid. The color intensity is inversely proportional to the concentration of the progesterone in the specimen and can be read at 450 nm.

High-Sensitivity Competitive ELISA for Progesterone

This ELISA kit enables sensitive and reproducible quantification of progesterone in biological samples, making it ideal for hormone level studies. Its competitive format and high-quality reagents ensure accurate results down to less than 0.05 ng/mL. Designed for research use, the kit provides everything required, minimizing hands-on time and potential technical errors.

User-Friendly Workflow and Fast Results

Thanks to its user-friendly design and carefully pre-formulated reagents, this kit streamlines your laboratory process. With a simple protocol and approximately 2-hour total assay time, researchers can obtain reliable progesterone measurements without extensive procedural complications. All materials arrive in standard-sized packaging and are easy to store at 28C.

Reliable and Traceable Calibration

The kit includes a comprehensive standard curve, enabling precise quantification across a broad range from 0.05 to 50 ng/mL. Calibration is traceable to international standards, supporting data comparability and scientific confidence in results. Additionally, accuracy is supported with typically less than 10% intra- and inter-assay CV.

FAQs of Progesterone ELISA Kit:

Q: How does the Progesterone ELISA Kit quantify hormone levels in samples?

A: The kit utilizes a competitive ELISA format, where sample progesterone competes with a labeled analogue for binding to specific antibodies. Results are quantified by comparing sample absorbance to the provided standard curve.

Q: What types of samples can be tested using this ELISA kit?

A: This kit is optimized for the quantitative determination of progesterone in serum or plasma samples. Each test requires only 50 L per well, making it suitable for limited-volume specimens.

Q: When is the best time to perform the assay after sample collection?

A: For optimal results, process samples shortly after collection or store them appropriately to prevent hormone degradation. Follow laboratory best practices and refer to the kits instructions regarding storage conditions prior to use.

Q: Where should the kit and its components be stored for maximum stability?

A: The complete kit and all reagents should be stored at 28C. Proper storage ensures a shelf life of up to 12 months from the date of manufacture.

Q: What is the process for calibrating and verifying the assay accuracy?

A: The kit includes standard solutions to generate a calibration curve for each run. Calibration is traceable to international standards, and results should be interpreted by comparing sample readings to this curve for quantitative analysis.

Q: How is the assay performed and read?

A: Manual operation of the assay is required, typically using a laboratory microplate reader to measure absorbance. All reagents are provided ready-to-use, and the protocol is straightforward, requiring basic laboratory equipment.

Q: What are the main benefits of using this Progesterone ELISA Kit?

A: Key benefits include high sensitivity and specificity, user-friendly workflow, ready-to-use reagents, fast assay time (about 2 hours), and reliable, internationally traceable calibration. The kit is ideal for research laboratories needing reproducible, quantitative progesterone measurements.

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