Glucose : The test is based on a double sequential enzyme reaction
One enzyme, glucose oxidase, catalyzes the formation of gluconic acid
and hydrogen peroxide with O-Toulident chromogen to oxidze the
chromogen to color s ramging from yellow to dark green.
Protein : The test is based on the protein error-of-indicators principle.
At a constant Ph, the development
of any green color is due to the presence of protein. Colors range from
green to green-blue for Positive reaction.