The H. pylori Ag Cassette Rapid Test is a sandwich lateral chromato graphic immuno as say. The test strip consists of:
A burgundy colored conjugate pad containing monoclonal anti-H. pylori antibody conjugated with colloidal gold (anti- H.p. conjugates) and a nitrocellulose membrane strip containing a test line (T line) and a control line (C line). The T line is pre-coated with another monoclonal anti-H. pylori antibody, and the C line is pre-coated with goat anti-mouse
IgG antibody.
When an adequate volume of extracted fecal specimen is dispensed into the sample well of the test cassette, the specimen migrates by capillary action across the cassette. H. pylori antigens, if present in the specimen, will bind to the anti-H.p. conjugates. The immuno complex is then captured on the membrane by the pre-coated antibody forming a burgundy colored T line, indicating an H. pylori positive test result. Absence of the T line suggests that the concentration of H. pylori antigens in the specimen is below the detectable level, indicating an H. pylori negative test result. The test contains an internal control (C line) which should exhibit a burgundy colored line of the immuno complex of goat anti-mouse IgG/mouse IgG-gold conjugate regardless of the color development on the T line. If the C line does not develop,