About Hepatitis E Virus IgG (HEV IgG) ELISA Kit
This kit employs solid phase, indirect ELISA method for detection of IgG antibodies to HEV (anti-HEV) in serum or plasma with two-step incubation procedure. Polystyrene micro well are pre-coated with purified activated recombinant HEV antigen. The HRP conjugated mouse anti human IgG (r chain) monoclonal antibody serves as tracer. TMB is substrate for HRP. The enzyme reaction with substrate TMB produces a color change, and the intensity of the absorbance at 450 nm indicates the presence or absence of Anti-HEV antibodies IgM in the sample. The test is specific, sensitive, reproducible and easy to operate. It is for blood screen of HEV infection.
Rapid and Reliable HEV IgG TestingThis ELISA kit provides quick results for the detection of Hepatitis E Virus IgG antibodies, streamlining the diagnostic workflow. Designed for manual operation, the kit includes all necessary reagents and controls, ensuring precise measurement and confidence in results. Its efficient testing cycle, with an assay time of just 6090 minutes, makes it an ideal choice for both routine and urgent analyses.
User-Friendly Design with Comprehensive ControlsWith a pre-coated microplate and ready-to-use reagents, this kit simplifies the testing process in clinical or research laboratories. Positive and negative controls are included for calibration and quality assurance, supporting reproducible and consistent outcomes. The clear instructions and standard components accommodate both experienced professionals and those new to ELISA technology.
Optimal Storage and Long Shelf LifeThe HEV IgG ELISA Kit maintains its efficacy when stored refrigerated between 28C, protecting the stability of reagents and extending usability. The kit is engineered for durability and safety, with a minimum shelf life of 1218 months from delivery when sealed and kept under recommended conditions. This ensures convenient stock management for laboratories.
FAQs of Hepatitis E Virus IgG (HEV IgG) ELISA Kit:
Q: How do I use the HEV IgG ELISA Kit for detecting Hepatitis E Virus antibodies?
A: To detect HEV IgG antibodies, add 1050 L of human serum or plasma to each well of the pre-coated microplate, following the provided protocol. The assay is manual and utilizes ready-to-use reagents. Run the reaction for 6090 minutes, then measure results using the colorimetric ELISA detection method.
Q: What are the recommended sample types and volumes for this ELISA kit?
A: This kit is optimized for human serum or plasma samples. Each test requires a sample volume of 1050 L per well, ensuring efficient use of material and high sensitivity for target detection.
Q: When should I use the controls provided in the HEV IgG ELISA Kit?
A: Positive and negative controls must be included in every assay run. They are essential for validating test accuracy, monitoring reagent performance, and ensuring the reliability of your results.
Q: Where should I store the HEV IgG ELISA Kit and its components?
A: Store the complete kit and all reagents in a refrigerator at 28C. Avoid freezing, as this could compromise reagent stability and assay performance. Proper storage extends the kits shelf life to 1218 months.
Q: What is the process flow and time required for the assay?
A: The ELISA process includes sample addition, incubation, washing, reagent addition, and colorimetric reading. The total assay time is typically 6090 minutes, providing rapid and efficient antibody detection.
Q: What are the main benefits of using this kit for HEV IgG detection?
A: The kit offers high sensitivity and specificity (95%), rapid results, reliable internal controls, and user-friendly components. It supports accurate diagnosis and monitoring of Hepatitis E Virus infections in clinical settings.