Beta HCG ELISA Kit

Beta HCG ELISA Kit Specification

Temperature Resistance
Store at 2-8C

Accuracy
High sensitivity and specificity

Features
Ready to use, Pre-coated wells, Easy to use protocol

Shape
Rectangular Microplate

Control Type
Manual (following kit instructions)

Type
ELISA Kit

Equipment Type
Diagnostic Kit

Material
Plastic, Pre-coated Microplate

Application
Quantitative detection of Beta HCG (Human Chorionic Gonadotropin) in serum or plasma

Reading Method
Photometric, preferably at 450 nm

Components Included
Pre-coated microplate, standard, conjugate, substrate, stop solution, wash buffer

Incubation Time
~2 hours total

Assay Format
96 wells

Sensitivity
Typically <2 mIU/mL

Detection Method
Sandwich ELISA, colorimetric detection

Regulatory Status
IVD/Research Use (Check package for details)

Intended Use
For laboratory and research use only

Shelf Life
12-18 months (unopened)

Sample Type
Serum or Plasma

Detection Range
Varies by kit (commonly 5-1000 mIU/mL)

Beta HCG ELISA Kit Trade Information

Minimum Order Quantity
1 Kit

Supply Ability
10000 Kits Per Week

Delivery Time
7 Days

Sample Policy
If order is confirmed we will reimburse the sample cost

Main Domestic Market
All India

About Beta HCG ELISA Kit

hCG is a one-step immunoassay, based on the principle of the sandwich method. The assay system utilizes high affinity and specificity monoclonal antibodies (enzyme conjugated and immobilized) directed against a distinct antigenic determinant on the beta sub unit of hCG molecule. The test sample is allowed to react simultaneously with the two antibodies, resulting in the hCG molecules being sandwiched between the solid phase and enzyme-linked antibodies. The unbound components are removed by washing. After addition of the solution containing TMB and hydrogen peroxide, the wells with bound conjugate develop a blue color which is converted to yellow after the reaction has been stopped with sulphuric acid. The color intensity is directly proportional to the concentration of hCG in the specimen and can be read at 450 nm.

Convenient and Efficient HCG Quantification

Designed for laboratories, the Beta HCG ELISA Kit enables quantitative analysis of Beta HCG in serum or plasma with minimal hands-on time. The pre-coated 96-well format, included standards, and straightforward protocol make it suitable for high-throughput or routine workflows.

High Sensitivity and Reliable Results

With a detection threshold of less than 2 mIU/mL and a broad dynamic range, this kit delivers robust and reproducible results. Its sandwich ELISA design ensures accurate assessment, reducing the chances of cross-reactivity and false positives in clinical and research settings.

FAQs of Beta HCG ELISA Kit:

Q: How does the Beta HCG ELISA Kit detect Beta HCG in samples?

A: The kit utilizes the sandwich ELISA principle where Beta HCG in serum or plasma binds between capture antibodies on the pre-coated microplate and detection antibodies. A colorimetric substrate is then added, and the intensity is measured photometrically at 450 nm to quantify the amount of HCG present.

Q: What types of samples can be analyzed with this ELISA kit?

A: This kit is designed specifically for human serum or plasma samples, enabling accurate quantitative measurement of Beta HCG in these biological fluids.

Q: When should the Beta HCG ELISA Kit be used in a laboratory?

A: The kit is suitable for use in both research and clinical diagnostic laboratories whenever accurate, quantitative detection of Beta HCG is required, such as in pregnancy-related studies or research on chorionic gonadotropin levels.

Q: Where should the kit be stored to ensure optimal performance and shelf life?

A: For best results, all components should be stored at 28C in their unopened condition, preserving the kits integrity and function for its stated shelf life of 1218 months.

Q: What is the typical detection range and sensitivity of the kit?

A: Most versions of the Beta HCG ELISA Kit can accurately measure Beta HCG concentrations from 5 to 1000 mIU/mL, with a sensitivity usually below 2 mIU/mL, ensuring detection of even low hormone levels.

Q: How is the assay performed, and how long does it take?

A: Following the manual protocol, samples and reagents are added to the pre-coated wells, incubated, washed, and reacted with substrate before reading. The complete process typically requires about two hours from start to finish.

Q: What are the main benefits of using this ELISA kit?

A: The benefits include high sensitivity and specificity, ease of use with ready-to-use reagents, manual control for flexibility, reliable quantification, and compatibility with standard laboratory microplate readers.

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