Salmonella typhi (S. typhi) is a gram-negative bacterium and it is the causative agent of typhoid fever, a characteristic severe septic illness seen exclusively in humans. In annual, an estimated 16.6 million new infections and 600,000 associated deaths occur. Typhoid fever can be diagnosed by isolation of S. typhi from blood, urine, or stool but this method requires laboratory equipments, technical training and time-consuming procedures. Widal test is used for serodiagnosis of typhoid fever but it also has many limitations on the interpretation. Biogenix Typhoid IgM/IgG is a simple and rapid laboratory test, which can S. typhi (not to S. paratyphi species) in whole blood. The nitrocellulose membrane of the kit immobilized with anti-human IgM (test line 1), anti-human IgG (test line 2), and goat anti-mouse antibodies (control line). And also, S. typhi conjugated to the colloidal gold particles, respectively. These conjugates are placed on a polyester or glass pad as conjugate pad. When the sample is dropped into the sample well on the device, the solubilized conjugate sample come into contact with the antibodies that immobilized onto the nitrocellulose. If the sample contains antibodies against S. typhi the result is visible as red line within ~20 minutes in the test line 1 (IgM) and/or in test line 2 (IgG) on the membrane. The solution continues to migrate to encounter a control reagent that binds a control conjugate, thereby producing another red control line.