Vitamin B12 ELISA Kit

Vitamin B12 ELISA Kit Specification

Features
Ready-to-use reagents, high accuracy, reliable results, simple procedur

Accuracy
High sensitivity, detection limit < 50 pg/mL

Temperature Resistance
Store at 28C

Display Type
Depends on microplate reader (not included)

Shape
Box

Control Type
Manual/Automated (compatible with ELISA reader)

Type
ELISA Kit

Dimension (L*W*H)
210 mm x 120 mm x 70 mm (box packaging)

Equipment Type
Biochemistry Diagnostic Kit

Equipment Materials
Plastic, polystyrene microplate

Material
Kit components include buffers, reagents, microplate strips

Application
For quantitative determination of Vitamin B12 in human serum and plasma

Vitamin B12 ELISA Kit Trade Information

Minimum Order Quantity
1 , , Kit

Sample Policy
If order is confirmed we will reimburse the sample cost

Main Domestic Market
All India

About Vitamin B12 ELISA Kit

Vitamin B12, also called cobalamin, is a water-soluble vitamin that has a key role in the normal functioning of the nervous system via the synthesis of myelin (myelinogenesis),(1, 2) and in the maturation of developing red blood cells in the bone marrow. It is involved in the metabolism of every cell of the human body: it is a co factor in DNA synthesis, fatty acid metabolism, and amino acid metabolism.(3) Vitamin B12 deficiency can potentially cause severe and irreversible damage, especially to the brain and nervous system.(4) At levels only slightly lower than normal, a range of symptoms such as fatigue, lethargy, depression, poor memory, breathlessness, headaches, and pale skin, among others, may be experienced, especially in elderly people (over age 60)(5, 6) who produce less stomach acid as they age, thereby increasing their probability of B12 deficiencies.(7) Vitamin B12 deficiency can also cause symptoms of mania and psychosis.(8) Vitamin B12 deficiency is most commonly caused by low intakes, but can also result from malabsorption, certain intestinal disorders, low presence of binding proteins, and use of certain medications. Vitamin B12 is rare from plant sources, so vegetarians are more likely to suffer from vitamin B12 deficiency. Infants are at a higher risk of vitamin B12 deficiency if they were born to vegetarian mothers. The elderly who have diets with limited meat or animal products are vulnerable populations as well. Vitamin B12 deficiency may occur in between 40% to 80% of the vegetarian population who are not also consuming a vitamin B12 supplement. (9) In Hong Kong and India, vitamin B12 deficiency has been found in roughly 80% of the vegan population as well.(10)

Advanced Competitive ELISA Technology

This kit employs a competitive ELISA method using a polystyrene microplate and pre-optimized buffers to deliver precise results. Its calibration against the WHO International Standard ensures accurate measurement of Vitamin B12 concentrations in the range of 1003000 pg/mL. Compatible with most ELISA readers for flexible testing options.

Convenient & Reliable Diagnostic Solution

Ready-to-use reagents and simplified protocols reduce preparation time and minimize error risk. Each kit supports both manual and automated processes, accommodating laboratories of various capacities. The high accuracy and low detection limit make it a preferred choice for clinical diagnostics and research applications.

FAQs of Vitamin B12 ELISA Kit:

Q: How is the Vitamin B12 ELISA Kit used for sample analysis?

A: The kit is designed to quantify Vitamin B12 in human serum or plasma samples using a competitive ELISA method. Users simply follow the provided protocoladding samples, standards, and reagents to the microplatethen measuring absorbance with a compatible ELISA reader.

Q: What are the storage requirements and shelf life of the Vitamin B12 ELISA Kit?

A: All kit components must be stored at 28C and must not be frozen. When stored as directed, the shelf life is 12 months, ensuring reagent stability and consistent results throughout the period.

Q: Where can the Vitamin B12 ELISA Kit be applied?

A: This kit is suitable for use in clinical laboratories, research institutes, hospitals, and diagnostic centers seeking quantitative measurement of Vitamin B12 in human serum or plasma samples.

Q: What process is involved in running an assay with this kit?

A: Performing the assay involves sample and reagent addition to the microplate, followed by incubation (approximately 120 minutes), washing, and reading absorbance values with a microplate reader, after which results are calculated based on the provided standard curve.

Q: What are the benefits of using this ELISA kit for Vitamin B12 detection?

A: The kit offers high sensitivity, accuracy, and reliability, with a low detection limit (< 50 pg/mL). Ready-to-use reagents and a straightforward procedure yield dependable results quickly, making it ideal for routine and high-throughput testing.

Q: When should this kit be chosen over other types of assays?

A: This kit is preferable when precise, quantitative analysis of Vitamin B12 levels in serum or plasma is required, especially in clinical or research settings demanding high sensitivity and standardized calibration.

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